Granzyme B （GrB） is a serine protease pivotal in cytotoxic immune responses, mediating apoptosis and immune surveillance. Structurally, GrB is synthesized as a 32 kDa inactive pro-enzyme （zymogen） and activated through proteolytic cleavage. Its active form features a conserved catalytic triad （His57, Asp102, Ser195） essential for enzymatic activity. Post-translational modifications, including N-linked glycosylation, enhance its stability and regulate intracellular trafficking. GrB functions by binding to mannose-6-phosphate receptors （MPRs） for endosomal uptake into target cells, where it synergizes with perforin to induce cell death.
Advantages of this OneStep ELISA Kit Design:
Simplified Workflow: Combines sample incubation and detection in a single step, eliminating intermediate washing and reducing hands-on time.
High Efficiency: Achieves results within 60 minutes, ideal for high-throughput studies or urgent diagnostics.
Enhanced Precision: Minimizes procedural variability and cross-contamination risks through optimized reagent formulation.
Broad Compatibility: Validated for diverse biological matrices, ensuring flexibility in experimental design.
This kit’s streamlined protocol and robust performance make it an indispensable tool for researchers and clinicians seeking reliable Granzyme B quantification in time-sensitive or resource-limited settings.